Detection of Somatic mutation of codon 248 of p53 gene between Iranian women with breast cancer


avatar Nasrin Shojaie 1 , * , avatar F. Tirgari 2

1 Department of Medical biochemistry, Iran Pasture Institute, Karaj, Iran

2 Department of Pathology, Imam Khomeini cancer Institute, Imam Khomeini hospital, Tehran, Iran

How to Cite: Shojaie N, Tirgari F. Detection of Somatic mutation of codon 248 of p53 gene between Iranian women with breast cancer. Int J Cancer Manag. 2008;1(1):e80392.


International Journal of Cancer Management: 1 (1); e80392
Published Online: January 30, 2008
Article Type: Research Article
Received: January 30, 2008
Accepted: January 30, 2008


Objective: Detection of P53 gene mutation on its codon 248 between Iranian women with breast cancer by PCR-RFLP.

Material and methods: DNA extraction of tumor tissues was performed in 53 patients with primary breast cancer. Designing primer for the sub-studying codon was performed with NTIVector software and detection of codon 248 mutation was done by Msp I PCR_RFLP.

Results: The somatic mutations of P53 on codon 248 were observed in 11.3% (6 of 53) of our cases, in which 9.4% were heterozygote mutation and the rest were homozygote mutation. This ratio is the highest reported percentage in the world.

Conclusion: This perusal indicates genotyping of P53 gene on codon 248 is important for Iranian women with breast cancer risk factors.


Full text is available in PDF.

© 2008, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.